Berberine mediates root remodeling in an immature tooth with apical periodontitis by regulating stem cells from apical papilla differentiation / 国际口腔科学杂志·英文版

Once pulp necrosis or apical periodontitis occurs on immature teeth, the weak root and open root apex are challenging to clinicians. Berberine (BBR) is a potential medicine for bone disorders, therefore, we proposed to apply BBR in root canals to enhance root repair in immature teeth. An in vivo model of immature teeth with apical periodontitis was established in rats, and root canals were filled with BBR, calcium hydroxide or sterilized saline for 3 weeks. The shape of the roots was analyzed by micro-computed tomography and histological staining. In vitro, BBR was introduced into stem cells from apical papilla (SCAPs). Osteogenic differentiation of stem cells from apical papilla was investigated by alkaline phosphatase activity, mineralization ability, and gene expression of osteogenic makers. The signaling pathway, which regulated the osteogenesis of SCAPs was evaluated by quantitative real time PCR, Western blot analysis, and immunofluorescence. In rats treated with BBR, more tissue was formed, with longer roots, thicker root walls, and smaller apex diameters. In addition, we found that BBR promoted SCAPs osteogenesis in a time-dependent and concentration-dependent manner. BBR induced the expression of β-catenin and enhanced β-catenin entering into the nucleus, to up-regulate more runt-related nuclear factor 2 downstream. BBR enhanced root repair in immature teeth with apical periodontitis by activating the canonical Wnt/β-catenin pathway in SCAPs.

The clinical observation and prognosis of indwelling double J tube by cystoscope for pregnancy with ureteral calculi / 中国基层医药

Objective To explore the effect of the cystoscope indwelling double J tube for the treatment of pregnancy with ureteral calculi,and to observe and analyze the prognosis,and provide the basis for clinical therapy. Methods 46 pregnant women with ureteral calculi were selected.All patients were treated by cystoscope indwelling double J tube.Observation indexes were the follow -up complications after a week of operation time,operation situa-tion,Visual analogue scale (VAS)was used to evaluate the degree of pain operation before and after treatment 48 h. To observe the pregnant women with double J tube placement and delivery status.Results 46 patients were success-fully catheter,the average operation time was (29.14 ±3.27)min.37 patients with lumbar cramps and fever and other symptoms were relieved after treatment.6 patients with replacement of double J tube 2 months later.After treatment, VAS score was (1.16 ±0.83)points,which was lower than (6.93 ±1.02)points before treatment,the difference was statistically significant (t =9.64,P <0.05).After treatment,the infection rates of hydronephrosis and urinary tract were 4.35% (2 /46)and 0% (0 /46),which were lower than before treatment [(15 /46,32.61%)and 19.57%(9 /46)],the differences were statistically significant (χ2 =8.33,9.05,all P <0.05);46 cases of pregnant women were smooth production,no abortion complications.The average indwelling double J tube time was (5.08 ± 1.16)months.Conclusion Cystoscope indwelling double J tube for the treatment of ureteral calculi in pregnancy has less invasion,high stone clearance rate,less postoperative complications and high safety,the prognosis was good,and it is a safe and effective method.

Non-open operation approach for the treatment of bening prostatic hyperplasia (BPH) complicated with Big Bladder Stones / 中国微创外科杂志

Objective To evaluate the mini-invasion approach for the treatment of BPH complicated with big bladder stones. Methods The clinical data of 30 cases of BPH complicated with big bladder stones treated by ESWL,mechanical lithotripsy using the visual lithotriptoscope and TURP or TUVP were retrospectively reviewed. Results 29 cases of big bladder stones were cleared up completely,and all of them were operated on successfully by TURP of TUVP.The operation time was short with minimal invasion The results were satisfactory.No severe complications and bladder stones have recurred up to now. Conclusions ESWL combined with mechanical lithotropsy using the visual lithotroptoscopy and TURP or TUVP is an effective.and non-open operation approach with mini-invasion for the treatment of BPH with big bladder stones.It applys to old patients with cardiovascular and pulmonary conditions,which would elevate the risk of operation.Proficiency in the technique is a key to a successful operation.